Cell Encapsulation in 3D Elastin-like Protein Hydrogels
7:11
Immunocytochemistry of Cells in 3D ELP Hydrogels
9:47
Results: Purification of Elastin-like Protein and its Application for 3D Hydrogel Cell Encapsulation
11:18
Conclusion
Transkript
The overall goal of this protocol is to express and purify recombinantly derived elastin-like proteins for use as a tunable hydrogel for 3D cell encapsulation. Additionally, this protocol presents the methodology for downstream fluorescent labelin
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Recombinant protein-engineered hydrogels are advantageous for 3D cell culture as they allow for complete tunability of the polymer backbone and therefore, the cell microenvironment. Here, we describe the process of recombinant elastin-like protein purification and its application in 3D hydrogel cell encapsulation.