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JoVE Journal

Neuroscience

Author Produced

This content is Free Access.

Dissection and Immunohistochemistry of the Drosophila Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron

4.1K Views

11:02 min

February 12th, 2022

February 12th, 2022

4,073 Views

0:01

Introduction

1:23

Initial Fixation of Tissues

3:13

Removing Leg Cuticle and Antibody Staining

6:58

Mounting Legs

7:41

Results

10:37

Conclusion

Transkript

We describe a dissection technique that preserves the architecture of the neuromuscular junction and enables a detailed immunocytochemical study of motor neurons in the adult Drosophila leg.

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