Johannes B. Huppa

Continuous T cell receptor signaling required for synapse maintenance and full effector potential.

Dynamics of cell surface molecules during T cell recognition.

T-cell-antigen recognition and the immunological synapse.

Linking molecular and cellular events in T-cell activation and synapse formation.

T cell killing does not require the formation of a stable mature immunological synapse.

CD4 enhances T cell sensitivity to antigen by coordinating Lck accumulation at the immunological synapse.

Quantitative imaging of lymphocyte membrane protein reorganization and signaling.

Agonist/endogenous peptide-MHC heterodimers drive T cell activation and sensitivity.

A hybrid machine-learning approach for segmentation of protein localization data.

TCR and Lat are expressed on separate protein islands on T cell membranes and concatenate during activation.

TCR-peptide-MHC interactions in situ show accelerated kinetics and increased affinity.

Evidence for a functional sidedness to the alphabetaTCR.

Functional development of the T cell receptor for antigen.

Photocrosslinkable pMHC monomers stain T cells specifically and cause ligand-bound TCRs to be 'preferentially' transported to the cSMAC.

Determination of interaction kinetics between the T cell receptor and peptide-loaded MHC class II via single-molecule diffusion measurements.

Distinct TCR signaling pathways drive proliferation and cytokine production in T cells.

The interdisciplinary science of T-cell recognition.

Rapid multiplex analysis of lipid raft components with single-cell resolution.

Assessment of costimulation and coinhibition in a triple parameter T cell reporter line: Simultaneous measurement of NF-κB, NFAT and AP-1.

RASGRP1 deficiency causes immunodeficiency with impaired cytoskeletal dynamics.

Förster Resonance Energy Transfer to Study TCR-pMHC Interactions in the Immunological Synapse.

A cellular platform for the evaluation of immune checkpoint molecules.

Monomeric TCRs drive T cell antigen recognition.

Mutations affecting the actin regulator WD repeat-containing protein 1 lead to aberrant lymphoid immunity.

Extracellular Purine Metabolism Is the Switchboard of Immunosuppressive Macrophages and a Novel Target to Treat Diseases With Macrophage Imbalances.

TCRs are randomly distributed on the plasma membrane of resting antigen-experienced T cells.

How drag sharpens a T cell's view on antigen.

Homo- and Heteroassociations Drive Activation of ErbB3.

Getting CD19 Into Shape: Expression of Natively Folded "Difficult-to- Express" CD19 for Staining and Stimulation of CAR-T Cells.

TIM-3 and CEACAM1 do not interact in cis and in trans.

Inefficient CAR-proximal signaling blunts antigen sensitivity.

The cytoskeletal regulator HEM1 governs B cell development and prevents autoimmunity.

Engineering AvidCARs for combinatorial antigen recognition and reversible control of CAR function.

Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy.

Functionalized Bead Assay to Measure Three-dimensional Traction Forces during T-cell Activation.

DNA origami demonstrate the unique stimulatory power of single pMHCs as T cell antigens.

A Multimodal Platform for Simultaneous T-Cell Imaging, Defined Activation, and Mechanobiological Characterization.

Histone deacetylase 1 controls CD4 T cell trafficking in autoinflammatory diseases.

SARS-CoV-2 mutations in MHC-I-restricted epitopes evade CD8 T cell responses.

Adjuvants and Vaccines Used in Allergen-Specific Immunotherapy Induce Neutrophil Extracellular Traps.

Temporal analysis of T-cell receptor-imposed forces via quantitative single molecule FRET measurements.

Characterization of CD8 T Cell-Mediated Mutations in the Immunodominant Epitope GP33-41 of Lymphocytic Choriomeningitis Virus.

Strategies for the Site-Specific Decoration of DNA Origami Nanostructures with Functionally Intact Proteins.

Three-Dimensional Single Molecule Localization Microscopy Reveals the Topography of the Immunological Synapse at Isotropic Precision below 15 nm.