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Affinity Chromatography Mediated Fibrinolytic Enzymes Purification from Crude Protein Extracts (Video) | JoVE

DOI :

10.3791/200167-v

•

June 2nd, 2023

June 2nd, 2023

•
375 Views
1Medicine School, Huaqiao University, 2Engineering Research Centre of Molecular Medicine of Ministry of Education

Tags

Affinity Chromatography
Fibrinolytic Enzymes
Purification
Crude Protein Extracts
Filter Membrane
Sample Storage
Arginine Agarose Matrix
Lysine Agarose Matrix
Equilibration
Tris Hydrochloride Buffer
Protein Sample Loading
Column Washing
Elution With Sodium Chloride Solutions
Fraction Collection
Sample Concentration
Ultracentrifugal Filter
Temperature Storage

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