JoVE Logo
Centro de recursos académicos

Iniciar sesión

Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification

DOI :

10.3791/54458-v

November 16th, 2016

November 16th, 2016

12,979 Views

1Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, 2Department of Emergency Medicine, Indiana University of School of Medicine

A protocol for the production, purification, and use of enzyme packaged outer membrane vesicles (OMV) providing for enhanced enzyme stability for implementation across diverse applications is presented.

Tags

Protein Packaging

-- Vistas

Videos relacionados

article

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

article

Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells

article

Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli

article

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

article

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

article

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

article

Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles

article

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

article

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs

article

Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor

JoVE Logo

Privacidad

Condiciones de uso

Políticas

Investigación

Educación

ACERCA DE JoVE

Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados