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Isolating Small Extracellular Vesicles (SEVs) by Differential Ultracentrifugation (Video) | JoVE

DOI :

10.3791/200347-v

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June 30th, 2023

June 30th, 2023

•

386 Views

Jiale Cheng^1,2, Jiong Zhu^1,2, Yujiao Liu^2,3, Chen Yang^2,3, Yuehui Zhang², Yuchen Liu², Chaozhi Jin², Jian Wang^1,2

¹School of Basic Medical Sciences, Anhui Medical University, ²State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, ³School of Life Sciences, Hebei University

Tags

Small Extracellular Vesicles

Differential Ultracentrifugation

Ultrafiltration Membrane

Bone marrow derived Macrophages

DMEM Culture Medium

Carbon Dioxide Incubation

Phosphate buffered Saline

Fetal Bovine Serum

Centrifuge Tubes

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