Observation Chamber Fabrication for Imaging Single-Cell Suspensions and Magnetic Nanoparticles

To begin, place the glass slide with holes on a clean surface with the plasma-activated side facing upwards. Carefully remove the protective layer from one side of the double-sided adhesive tape in the direction of cutting. Align the tape with the edges of the glass slide and the drilled holes.

Place the tape on the glass slide starting from the short edge and make sure of good adherence. After removing the second protective layer from the tape, place the second glass slide with the activated surface facing downwards. Using a flat board, press the two glass slides together while applying the upper body strength for 10 seconds.

Flip the chamber so that the holes face the user. Apply a small amount of UV curable glue in the ring below the NanoPort. Slide the NanoPort down the hole in the glass slide using a microtubing and add a ring of UV curable glue around the port.

Load one milliliter of 1%fluorosilane solution prepared in fluorinated oil into a syringe. Inject the solution through a PTFE syringe filter and a needle attached to PTFE microtubing into the observation chamber. After a minute of incubation, use nitrogen pressure to flush out the coating solution under a fume hood.

Using a different syringe assembly, rinse the chamber solely with fluorinated oil.