Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells
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09:43 min
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March 8th, 2024
DOI :
March 8th, 2024
•Aline Linder*1, Kevin Portmann*1, Luca Johannes Schlotheuber1, Alessandro Streuli1, Wiona Sophie Glänzer1, Klaus Eyer1,2, Ines Lüchtefeld1,3
1Laboratory for Functional Immune Repertoire Analysis, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zürich, 2Department of Biomedicine, Aarhus University, 3Laboratory for Tumor and Stem Cell Dynamics, Institute of Molecular Health Sciences, Department of Biology, ETH Zürich
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Chapters in this video
0:00
Introduction
2:04
Observation Chamber Fabrication for Imaging Single‐Cell Suspensions and Magnetic Nanoparticles
3:50
Preparation of Functionalized Magnetic Nanoparticles for Time‐Resolved Fluorescent Imaging
5:33
Encapsulation of the Cells and Nanoparticles into Droplets for Fluorescent Imaging
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