June 21st, 2013
•Reconstituting functional membrane proteins into giant liposomes of defined composition is a powerful approach when combined with patch-clamp electrophysiology. However, conventional giant liposome production may be incompatible with protein stability. We describe protocols for producing giant liposomes from pure lipids or small liposomes containing ion channels.
Videos relacionados
Methods for Patch Clamp Capacitance Recordings from the Calyx
Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes
Pressure-polishing Pipettes for Improved Patch-clamp Recording
Preparation of Artificial Bilayers for Electrophysiology Experiments
Spinal Cord Electrophysiology
Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes
Exploring Arterial Smooth Muscle Kv7 Potassium Channel Function using Patch Clamp Electrophysiology and Pressure Myography
Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry
One-channel Cell-attached Patch-clamp Recording
Reconstitution of a Transmembrane Protein, the Voltage-gated Ion Channel, KvAP, into Giant Unilamellar Vesicles for Microscopy and Patch Clamp Studies
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados