Umbilical cords were collected with the assistance of the Birmingham Women's Health Care NHS Trust. HMM was supported by an Arthritis Research UK Career Development Fellowship (19899) and Systems Science for Health, University of Birmingham (5212).

<ol>
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N., Savill, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Resolution+of+inflammation:+the+beginning+programs+the+end.">Resolution of inflammation: the beginning programs the end.</a> Nature immunology. 6 (12), 1191-1197 (2005).</li><li>Schmidt, S., Moser, M., Sperandio, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+molecular+basis+of+leukocyte+recruitment+and+its+deficiencies.">The molecular basis of leukocyte recruitment and its deficiencies.</a> Molecular immunology. 55, 49-58 (2013).</li><li>Luu, N. T., Rainger, G. E., Nash, G. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differential+Ability+of+Exogenous+Chemotactic+Agents+to+Disrupt+Transendothelial+Migration+of+Flowing+Neutrophils.">Differential Ability of Exogenous Chemotactic Agents to Disrupt Transendothelial Migration of Flowing Neutrophils.</a> The Journal of Immunology. 164 (11), 5961-5969 (2000).</li><li>Smith, C. W., Rothlein, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recognition+of+an+endothelial+determinant+for+CD+18-dependent+human+neutrophil+adherence+and+transendothelial+migration.">Recognition of an endothelial determinant for CD 18-dependent human neutrophil adherence and transendothelial migration.</a> The Journal of clinical investigation. 82 (5), 1745-1756 (1988).</li><li>Luscinskas, F. W., Brock, A. F., Arnaout, M. A., Gimbrone, M. 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M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Omega-3+Fatty+acids+and+inflammation:+novel+interactions+reveal+a+new+step+in+neutrophil+recruitment.">Omega-3 Fatty acids and inflammation: novel interactions reveal a new step in neutrophil recruitment.</a> PLoS biology. 7 (8), e1000177 (2009).</li><li>Ahmed, S. R., McGettrick, H. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prostaglandin+D2+regulates+CD4++memory+T+cell+trafficking+across+blood+vascular+endothelium+and+primes+these+cells+for+clearance+across+lymphatic+endothelium.">Prostaglandin D2 regulates CD4+ memory T cell trafficking across blood vascular endothelium and primes these cells for clearance across lymphatic endothelium.</a> Journal of immunology. 187 (3), 1432-1439 (2011).</li><li>Bradfield, P. F., Amft, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rheumatoid+fibroblast-like+synoviocytes+overexpress+the+chemokine+stromal+cell-derived+factor+1+(CXCL12),+which+supports+distinct+patterns+and+rates+of+CD4++and+CD8++T+cell+migration+within+synovial+tissue.">Rheumatoid fibroblast-like synoviocytes overexpress the chemokine stromal cell-derived factor 1 (CXCL12), which supports distinct patterns and rates of CD4+ and CD8+ T cell migration within synovial tissue.</a> Arthritis and rheumatism. 48 (9), 2472-2482 (2003).</li><li>Filer, A., Parsonage, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differential+survival+of+leukocyte+subsets+mediated+by+synovial,+bone+marrow,+and+skin+fibroblasts:+site-specific+versus+activation-dependent+survival+of+T+cells+and+neutrophils.">Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.</a> Arthritis and rheumatism. 54 (7), 2096-2108 (2006).</li><li>Lally, F., Smith, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+mechanism+of+neutrophil+recruitment+in+a+coculture+model+of+the+rheumatoid+synovium.">A novel mechanism of neutrophil recruitment in a coculture model of the rheumatoid synovium.</a> Arthritis and rheumatism. 52 (11), 3460-3490 (2005).</li><li>Chakravorty, S. J., McGettrick, H. M., Butler, L. M., Buckley, C. D., Rainger, G. E., Nash, G. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+in+vitro.+model+for+analysing+neutrophil+migration+into+and+away+from+the+sub-endothelial+space:+Roles+of+flow+and+CD31.">An in vitro. model for analysing neutrophil migration into and away from the sub-endothelial space: Roles of flow and CD31.</a> Biorheology. 43 (1), 71-82 (2006).</li><li>Rainger, G. E., Nash, G. 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The authors declare that they have no conflicts of interest.

Aquí se describen los dos modelos &quot;in vitro&quot; en vasculares para estudiar el reclutamiento de neutrófilos circulantes por endotelio inflamado. Una gran ventaja de estos modelos es la capacidad de analizar cada paso en la cascada de adhesión de leucocitos en orden, como ocurriría in vivo. Hemos observado anteriormente un aumento dependiente de la dosis en la adhesión de neutrófilos y la transmigración a través de TNF estimulada CE 9,29. También describimos cómo ambos modelo...

La inflamación es una respuesta protectora a la infección microbiana o la lesión del tejido que requiere una estrecha regulación de la entrada de leucocitos y salida de el tejido inflamado para permitir la resolución 1,2. Cross-talk entre las células endoteliales (CE) que los vasos sanguíneos línea, leucocitos circulantes y las células del estroma residente de tejidos es esencial para coordinar este proceso 3. Sin embargo, la contratación incontrolado de leucocitos y su limpieza ineficaz de apuntalan el desarrollo de enfermedades inflamatorias crónicas 4. Nuestra comprensión actual de reclutamiento de leucocitos en la salud y la enfermedad es incompleta y se necesitan modelos más robustos para analizar este proceso. Los mecanismos de apoyo el reclutamiento de leucocitos de la sangre a través de CE vascular en las vénulas post-capilares han sido bien descritos 1,2,5. Leucocitos circulantes son capturados por los receptores especializados (por ejemplo, VCAM-1, E-selectina, P-selectina), queestán reguladas por el endotelio inflamado. Estas interacciones transitorias permiten leucocitos interactuar con quimiocinas unidas a la superficie y mediadores derivados de lípidos (ya sea endoteliales o estroma en origen) que activan las integrinas expresadas por los leucocitos 6- 11. Esto a su vez estabiliza la adhesión y la migración de las unidades a través del endotelio y en el tejido 12- 15. Dentro del tejido, reclutados leucocitos se someten a los agentes derivado del estroma que influyen en su motilidad, la función y supervivencia 16,17. La creciente evidencia sugiere que las señales recibidas en cada etapa de las condiciones del proceso de reclutamiento de leucocitos para la próxima. Sin embargo, nuestra comprensión de reclutamiento de leucocitos sigue siendo incompleta y muy poco se sabe sobre el movimiento de leucocitos componentes conformación dentro del tejido. En Birmingham hemos desarrollado varios modelos in vitro &quot;vasculares&quot; para estudiar el reclutamiento de leucocitos defluir 9,18,19. Ahora entendemos que vascular acto CE reguladores como inmediatos de reclutamiento de leucocitos en respuesta a los cambios en su microambiente local. Específicamente, las células del estroma de tejido residente pueden regular activamente la respuesta inflamatoria, en parte por conversar con vecinos CE vascular para influir en su papel en el reclutamiento 3. Hemos demostrado anteriormente que las diversas células del estroma modulan la capacidad de CE para apoyar la adhesión y migración de leucocitos en un tejido de manera específica, y que estos efectos se vuelven alterada en enfermedades crónicas 13,16,20,21. Así, las células del estroma de tejido establecen &#39;de direcciones códigos&#39; que definen el contexto de cada respuesta inflamatoria 22. Más recientemente, hemos demostrado que la médula ósea derivada MSC (BMMSC) potentemente regular a la baja la respuesta de la CE a las citoquinas, lo que lleva a una reducción en el reclutamiento de neutrófilos y linfocitos 23. Los mecanismos govreclutamiento sejo de dilucidado in vitro han utilizado en gran medida ensayos que incorporan un solo tipo de células (por ejemplo, CE) o la proteína aislada. Sin embargo, estos estudios no tienen en cuenta los efectos del entorno local de los tejidos (es decir, la presencia de células del estroma) en el reclutamiento de leucocitos y su posterior migración en el tejido. Aquí se describen dos métodos basados ​​en el flujo en el que las células del estroma, específicamente las células madre mesenquimales (MSC), somos co-cultivadas con CE 23. Estos modelos nos permiten examinar el efecto de las células del estroma en las respuestas endoteliales, en particular su capacidad para apoyar el reclutamiento de leucocitos de flujo.

<table border="0" cellpadding="0" cellspacing="0" >
	<tbody>
		<tr >
			<td >Collagenase Type Ia</td>
			<td >Sigma</td>
			<td>C2674</td>
			<td>Dilute in 10ml PBS to get a final concentration of 10mg/ml. Store at -20&#176;C in 1ml aliquots.</td>
		</tr>
		<tr >
			<td >Dulbecco&#39;s PBS</td>
			<td >Sigma</td>
			<td >D8662</td>
			<td>With calcium and magnesium chloride. Keep sterile and store at room temperature.</td>
		</tr>
		<tr >
			<td >1X Medium M199</td>
			<td >Gibco</td>
			<td >31150-022</td>
			<td>Warm in 37 &#176;C water bath before use.</td>
		</tr>
		<tr >
			<td >Gentamicin sulphate</td>
			<td >Sigma</td>
			<td >G1397</td>
			<td>Store at 4&#176;C. Add to M199 500ml bottle.</td>
		</tr>
		<tr >
			<td >Human epidermal growth factor</td>
			<td >Sigma</td>
			<td >E9644</td>
			<td>Store at -20&#176;C in 10&#181;l aliquots.</td>
		</tr>
		<tr >
			<td >Fetal calf serum (FCS)</td>
			<td >Sigma</td>
			<td >F9665</td>
			<td >FCS must be batch tested to ensure the growth and viability of isolated EC. Heat inactivate at 56&#176;C. Store in 10ml aliquots at -20&#176;C.</td>
		</tr>
		<tr >
			<td >Amphotericin B</td>
			<td >Gibco</td>
			<td >15290-026</td>
			<td >Potent and becomes toxic within a week so fresh complete HUVEC medium must be made up every week. Store at -20&#176;C in 1ml aliquots.</td>
		</tr>
		<tr >
			<td >Hydrocortisone</td>
			<td >Sigma</td>
			<td >H0135</td>
			<td>Stock is in ethanol. Store at -20&#176;C in 10&#181;l aliquots.</td>
		</tr>
		<tr >
			<td >Collagenase Type II</td>
			<td >Sigma</td>
			<td >C6885</td>
			<td>Dilute stock in PBS to a final concentration of 100mg/ml. Store at -20&#176;C in 100&#181;l aliquots.</td>
		</tr>
		<tr >
			<td >Hyaluronidase</td>
			<td >Sigma</td>
			<td >H3631</td>
			<td>Dilute stock in PBS to a final concentration of 20,000U/ml. Store at -20&#176;C in 100&#181;l aliquots.</td>
		</tr>
		<tr >
			<td >100&#181;m cell strainer for 50ml centifuge tube</td>
			<td >Scientific Lab Supplies (SLS)</td>
			<td >352360</td>
			<td >Other commercially available cell strainers (e.g. Greiner bio-one) can also be used.</td>
		</tr>
		<tr >
			<td >DMEM low glucose</td>
			<td >Biosera</td>
			<td >LM-D1102/500</td>
			<td>Warm in 37 &#176;C water bath before use.</td>
		</tr>
		<tr >
			<td >Penicillin/Streptomycin mix</td>
			<td >Sigma</td>
			<td >P4333</td>
			<td>Store at -20&#176;C in 1ml aliquots.</td>
		</tr>
		<tr >
			<td >25cm2 tissue culture flask</td>
			<td >SLS</td>
			<td >353109</td>
			<td></td>
		</tr>
		<tr >
			<td >75cm2 tissue culture flask</td>
			<td >SLS</td>
			<td >353136</td>
			<td></td>
		</tr>
		<tr >
			<td >Bone marrow mesenchymal stem cells vial</td>
			<td >Lonza</td>
			<td >PT-2501</td>
			<td >Store in liquid nitrogen upon arrival. Cells are at passage 2 upon arrival but are designated passage 0. Exapand to passage 3 and store in liquid nitrogen for later use.</td>
		</tr>
		<tr >
			<td >Mesenchymal stem cell growth medium (MSCGM)</td>
			<td >Lonza</td>
			<td >PT-3001</td>
			<td>Warm in 37 &#176;C water bath before use. For Cell Tracker Green staining use medium without FCS.</td>
		</tr>
		<tr >
			<td >EDTA (0.02%) solution</td>
			<td >Sigma</td>
			<td >E8008</td>
			<td>Store at 4&#176;C. Warm in 37&#176;C water bath before use.</td>
		</tr>
		<tr >
			<td >Trypsin solution</td>
			<td >Sigma</td>
			<td >T4424</td>
			<td>Store at -20&#176;C in 2ml aliquots. Thaw at room temperature and use immediately.</td>
		</tr>
		<tr >
			<td >Cryovials</td>
			<td >Greiner bio-one</td>
			<td>2019-02</td>
			<td>Keep on ice before adding before adding cell suspension.</td>
		</tr>
		<tr >
			<td >Mr. Frosty Freezing Container</td>
			<td >Nalgene</td>
			<td >5100-0001</td>
			<td >Store at room temperature. When adding cryovials with cells store at -80&#176;C for 24h before transfrring cells to liquid nitrogen.</td>
		</tr>
		<tr >
			<td >Ibidi u-Slide VI (0.4), T/C treated, sterile</td>
			<td >Ibidi</td>
			<td >IB-80606</td>
			<td >Alternative models include glass capillaries, Cellix Biochips (www.cellixltd.com), BioFlux Plates (www.fluxionbio.com/bioflux/) and GlycoTech parallel plate flow chambers (http://www.glycotech.com/apparatus/parallel.html).</td>
		</tr>
		<tr >
			<td >Cell tracker green dye</td>
			<td >Life technologies</td>
			<td >C2925</td>
			<td>Store in 5&#181;l aliquots at -20&#176;C. Dilute in 5ml prewarmed (at 37&#176;C) MSCGM.</td>
		</tr>
		<tr >
			<td >Cell counting chambers</td>
			<td >Nexcelom</td>
			<td >SD-100</td>
			<td>Alternatively a haemocytometer can be used.</td>
		</tr>
		<tr >
			<td >Cellometer auto T4 cell counter</td>
			<td >Nexcelom</td>
			<td >Auto T4-203-0238</td>
			<td></td>
		</tr>
		<tr >
			<td >Tumor necrosis factor &#945; (TNF&#945;)</td>
			<td >R&#38;D Systems</td>
			<td >210-TA-100</td>
			<td>Dilute stock in PBS to a final concentration of 100,000U/ml. Store at -80&#176;C in 10&#181;l aliquots.</td>
		</tr>
		<tr >
			<td >6-well, 0.4&#181;m PET Transwell filters</td>
			<td >SLS</td>
			<td >353090</td>
			<td></td>
		</tr>
		<tr >
			<td >K2-EDTA in 10ml tubes</td>
			<td >Sarstedt</td>
			<td ></td>
			<td>Store at room temperature.</td>
		</tr>
		<tr >
			<td >Histopaque 1119</td>
			<td >Sigma</td>
			<td >11191</td>
			<td>Store at 4&#176;C. Warm to room temperature before use.</td>
		</tr>
		<tr >
			<td >Histopaque 1077</td>
			<td >Sigma</td>
			<td >10771</td>
			<td>Store at 4&#176;C. Warm to room temperature before use.</td>
		</tr>
		<tr >
			<td >10ml round bottomed tube</td>
			<td >Appleton Woods</td>
			<td >SC211 142 AS</td>
			<td></td>
		</tr>
		<tr >
			<td >7.5% BSA Fraction V solution</td>
			<td >Life technologies</td>
			<td >15260-037</td>
			<td>Store at 4&#176;C.</td>
		</tr>
		<tr >
			<td >20ml Plastipak syringes</td>
			<td >BD falcon</td>
			<td >300613</td>
			<td></td>
		</tr>
		<tr >
			<td >5ml Plastipak syringes</td>
			<td >BD falcon</td>
			<td >302187</td>
			<td></td>
		</tr>
		<tr >
			<td >2ml Plastipak syringes</td>
			<td >BD falcon</td>
			<td >300185</td>
			<td></td>
		</tr>
		<tr >
			<td >3M hypo-allergenic surgical tape 9m x 2.5cm</td>
			<td >Micropore</td>
			<td >1530-1</td>
			<td>Use to secure the syringe tap onto the wall of the perspex chamber.</td>
		</tr>
		<tr >
			<td >Silicon rubber tubing, internal diameter/external diameter (ID/OD) of 1/3mm (thin tubing)</td>
			<td >Fisher Scientific</td>
			<td >FB68854</td>
			<td >Cut silicon tubing to the appropriate size. All tubing leading directly to the electronic microvalve must be thin.</td>
		</tr>
		<tr >
			<td >Silicon rubber tubing ID/OD of 2/4mm (thick tubing)</td>
			<td >Fisher Scientific</td>
			<td >FB68855</td>
			<td></td>
		</tr>
		<tr >
			<td >Portex Blue Line Manometer tubing</td>
			<td>Smiths</td>
			<td >200/495/200</td>
			<td>Tubing leading to the syringe pump.</td>
		</tr>
		<tr >
			<td >3-way stopcock</td>
			<td >BOC Ohmeda AB</td>
			<td ></td>
			<td></td>
		</tr>
		<tr >
			<td >Glass 50ml syringe for pump</td>
			<td >Popper Micromate</td>
			<td >550962</td>
			<td>Must be primed prior to use by removing any air bubbles.</td>
		</tr>
		<tr >
			<td >Glass coverslip</td>
			<td >Raymond A Lamb</td>
			<td ></td>
			<td>26x76mm coverslips made to order. Lot number 2440980.</td>
		</tr>
		<tr >
			<td >Parafilm gasket</td>
			<td >American National Can Company</td>
			<td ></td>
			<td >Cut a 26x76mm piece of parafilm using an aluminium template and cut a 20x4mm slot into it using a scalpel 10a. Gasket thickness is approximately 133&#181;m.</td>
		</tr>
		<tr >
			<td >Two perspex parallel plates</td>
			<td >Wolfson Applied Technology Laboratory</td>
			<td ></td>
			<td >Specially designed chamber consisting of parallel plates held together by 8 screws. The lower plate has a viewing slot cut out in the middle and a shallow recess milled to allow space for the coverslip, filter and gasket. The upper perspex plate has an inlet and outlet hole positioned over the flow channel.</td>
		</tr>
		<tr >
			<td >Electronic 3-way microvalve with min. dead space</td>
			<td >Lee Products Ltd.</td>
			<td >LFYA1226032H</td>
			<td>Electronically connected to a 12 volt DC power supply.</td>
		</tr>
		<tr >
			<td >Syringe pump for infusion/withdrawal (PHD2000)</td>
			<td >Harvard Apparatus</td>
			<td >70-2001</td>
			<td>Set the diameter to 29mm and refill (flow) rate.</td>
		</tr>
		<tr >
			<td >L-shaped connector</td>
			<td >Labhut</td>
			<td >LE876</td>
			<td>To attach to the inlet and outlet ports onto the Ibidi microslide channel.</td>
		</tr>
		<tr >
			<td >Video camera</td>
			<td >Qimaging</td>
			<td >01-QIC-F-M-12-C</td>
			<td>Connected to a computer which enables digitall videos to be recorded.</td>
		</tr>
		<tr >
			<td >Image-Pro Plus 7.0</td>
			<td >Media Cybernetics</td>
			<td >41N70000-61592</td>
			<td >For data analysis. Manually tag cells displaying the different behaviors. Track cells for analysis of rolling and migration velocities.</td>
		</tr>
		<tr >
			<td ></td>
			<td ></td>
			<td ></td>
			<td>Refer to product datasheets for details on hazards of using the reagents described here.</td>
		</tr>
	</tbody>
</table>

analyzing the effects of stromal cells on the recruitment of leukocytes from flow

Las células del estroma regulan el reclutamiento de leucocitos circulantes durante la inflamación a través de la diafonía con células endoteliales vecinas. Aquí se describen los dos modelos &quot;in vitro&quot; en vasculares para estudiar el reclutamiento de neutrófilos circulantes de flujo por las células endoteliales inflamadas. Una gran ventaja de estos modelos es la capacidad de analizar cada paso en la cascada de adhesión de leucocitos en orden, como ocurriría in vivo. También describimos cómo ambos modelos se pueden adaptar para estudiar el papel de las células del estroma, en este caso las células madre mesenquimales (MSC), en la regulación de reclutamiento de leucocitos. Células endoteliales primarias se cultivaron solas o junto con MSC humano en contacto directo en microportaobjetos ibidi o en lados opuestos de un filtro Transwell durante 24 horas. Los cultivos se estimularon con factor de necrosis tumoral alfa (TNFa) durante 4 horas y se incorporan en un ensayo de adherencia basado en el flujo. Un bolo de neutrófilos se perfundiósobre el endotelio durante 4 min. La captura de fluir neutrófilos y sus interacciones con el endotelio fue visualizado por microscopía de contraste de fase. En ambos modelos, la estimulación de citoquinas-aumento del reclutamiento de neutrófilos endotelial que fluyen de una manera dependiente de la dosis. Análisis del comportamiento de los neutrófilos reclutados mostró una disminución dependiente de la dosis en la rodadura y un aumento dependiente de la dosis en la transmigración a través del endotelio. En co-cultivo, MSC suprimida la adhesión de neutrófilos al endotelio TNF estimulada. Nuestros modelos de adhesión basados ​​en el flujo imitan las fases iniciales de reclutamiento de leucocitos de la circulación. Además de leucocitos, pueden usarse para examinar el reclutamiento de otros tipos de células, como las células tumorales circulantes MSC o administrados terapéuticamente. Nuestros modelos de co-cultivo de varias capas han demostrado que el MSC se comunica con el endotelio de modificar su respuesta a las citoquinas pro-inflamatorias, Altering el reclutamiento de neutrófilos. Se requiere investigación adicional utilizando dichos modelos para entender completamente las células estromales de la forma de diferentes tejidos y condiciones (trastornos inflamatorios o cáncer) influyen en el reclutamiento de leucocitos durante la inflamación.

Inicialmente, se analizó el efecto de estimular la CE con TNFa en el reclutamiento de neutrófilos de flujo utilizando el modelo microportaobjetos ibidi (Sección 7-9). En ausencia de TNF, poco o ningún neutrófilos se adhirieron a la monocapa endotelial (Figura 2A). Esto se esperaba, ya que sin tratar / descansando CE no expresan las moléculas necesarias de adhesión (selectinas) o quimiocinas para apoyar la unión 25,26. En contraste, la estimulación por citoquinas aume...

Watch this Scientific Journal Video about Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow at JoVE.com

Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow

La capacidad del endotelio inflamado para reclutar leucocitos de flujo se regula por las células estromales mesenquimales. Se describen dos modelos in vitro que incorporan células humanas primarias que se pueden utilizar para evaluar el reclutamiento de neutrófilos de flujo y examinar el papel que las células estromales mesenquimales desempeñan en la regulación de este proceso.

El análisis de los efectos de las células del estroma en el reclutamiento de leucocitos de Flujo

Stromal cells regulate the recruitment of circulating leukocytes during inflammation through cross-talk with neighboring endothelial cells. Here we ...

analyzing-effects-stromal-cells-on-recruitment-leukocytes-from

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JoVE Journal

Immunology and Infection

10.1K vistas.  University of Birmingham.  La capacidad del endotelio inflamado para reclutar leucocitos de flujo se regula por las células estromales mesenquimales. Se describen dos modelos in vitro que incorporan células humanas primarias que se pueden utilizar para evaluar el reclutamiento de neutrófilos de flujo y examinar el papel que las células estromales mesenquimales desempeñan en la regulación de este proceso. 