Isolation of Neutrophils from Peripheral Human Blood
2:17
Measurement of Kinetics of Extracellular DNA Release Using Microplate Fluorimeter
4:22
Quantitation of NET Formation by MPO-DNA and HNE-DNA ELISA Assays
8:22
Results: MPO-DNA and HNE-DNA ELISA Assay Results
10:25
Conclusion
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The overall goal of this procedure is to quantitate neutrophil extracellular traps, or NETs, released from human neutrophils on a high throughput scale. The methods presented here can help answer key questions in the fields of inflammation and alt
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High throughput assays are presented that in combination provide excellent tools to quantitate NET release from human neutrophils.