Confocal Microscopic Calibration of the Ratiometric Dye
3:31
Collection of In Situ-grown Dental Biofilm Samples
5:12
Biofilm pH Imaging
6:59
Digital Image Analysis
10:44
Results: Ratiometric Measuring of Extracellular pH in Dental Biofilms
12:16
Conclusion
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The overall goal of this Confocal Microscopy based Ratiometric Imaging Technique is to determine and visualize Extracellular pH in Bacterial Biofilms in real time. This method helps to answer some key questions in the field of Biofilm research suc
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A pH-sensitive ratiometric dye is used in combination with confocal laser scanning microscopy and digital image analysis to monitor extracellular pH in dental biofilms in real-time.