Final Approach, Seal Formation, and Obtaining the Whole-cell Configuration
5:56
Results: Effect of Temperature, Series Resistance, and Input Resistance on the Waveform of the Electrical Signal
6:47
Conclusion
Transcripción
The overall goal of this procedure is to describe how to perform whole-cell patch-clamp recordings in freshly dissected brain slices. This method can help answer key questions in the field of neuroscience, such has how experimental control manipul
Sign in or start your free trial to access this content
This protocol describes basic procedural steps for performing whole-cell patch-clamp recordings. This technique allows the study of the electrical behavior of neurons, and when performed in brain slices, allows the assessment of various neuronal functions from neurons that are still integrated in relatively well preserved brain circuits.