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Protocol for the Direct Conversion of Murine Embryonic Fibroblasts into Trophoblast Stem Cells

7.5K Views

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08:57 min

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July 25th, 2016

DOI :

10.3791/54277-v

July 25th, 2016

•
7,499 Views
1Department of Developmental Pathology, Institute of Pathology, University of Bonn Medical School

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Keywords Murine Embryonic Fibroblasts
Trophoblast Stem Cells
Induced Trophoblast Stem Cells
ITSCs
Transfection
Lentiviral Vector
293T Cells
Virus Production
Cell Culture
Poly L lysine
Chloroquine
HEPES Buffered Saline
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