The overall goal of this method is to effectively and incrementally manipulate dissolved oxygen or DO conditions in a laboratory setting. To allow the observation of aquatic animal behavioral responses. The neat thing about this method is it can h
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This article describes a simple and reproducible protocol to manipulate dissolved oxygen conditions in a laboratory setting for animal behavior studies. This protocol may be used in both teaching and research laboratory settings to evaluate organismal response of macroinvertebrates, fishes, or amphibians to changes in dissolved oxygen concentration.