Screening for Life Stage and Tissue of Interest with Live Imaging
6:20
Sample Preservation, Dehydration and Mounting for Electron Microscopy
8:00
Location a Calcium Rich Region Using SEM-EDS
9:11
Identifying Crystallographic Information of Calcium Rich Regions Using SEM-EBSD
10:28
TEM Sample Preparation Using FIB-SEM
12:32
Obtaining Selected Area Diffraction Pattern on a TEM
13:47
Representative Results
15:05
Conclusion
Transcripción
The overall goal of this procedure is to determine the location and structure of calcification event by performing a combination of optical and electron microscopy methods. This is accomplished by monitoring the living larval tube worm during meta
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We demonstrate the use of various microscopy methods that are useful in observing the calcification of a tubeworm, Hydroides elegans, as well as locating and characterizing the first calcified material. Live microscopy and electron microscopy are used together to provide functional and material information that are important in studying biomineralization.