Preparation of Cell Suspension from Embryonic Mouse Heart
5:31
Cardiac Cell Staining
6:54
Data Acquisition and Analysis
8:44
Results: Spectral Cytometry-based Characterization of Embryonic Cardiac Cells
9:50
Conclusion
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The overall goal of this spectral cytometry technique is to distinguish different fluorochromes using their entire emissions spectra. More over this protocol enables implementation of autofluorescence as an independent parameter, allowing for a pr
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This article describes spectral cytometry, a new approach in flow cytometry that uses the shapes of emission spectra to distinguish fluorochromes. An algorithm replaces compensations and can treat auto-fluorescence as an independent parameter. This new approach allows for the proper analysis of cells isolated from solid organs.