Preparation of Eggs and Microdissection of Chicken Spinal Neural Tube (SNT)
4:12
Crosslinking Proteins to DNA
5:01
Lysis, Sonication, and Immunoprecipitation
6:33
Washing, Elution, and Reversal of Crosslinking
8:57
Cellular Protein and RNA Digestion
10:06
Results: Evaluation of the ChIP Protocol by ChIP-seq Experiments
11:48
Conclusion
Transcribir
The overall goal of this procedure is to investigate the binding profiles of different histone modifications, using low-abundance embryonic samples in order to improve the functional annotation of vertebrate genomes. This method can help answer ke
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Here, we describe a chromatin immunoprecipitation (ChIP) and ChIP-seq library preparation protocol to generate global epigenomic profiles from low-abundance chicken embryonic samples.