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Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

12.2K Views

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10:59 min

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November 23rd, 2017

DOI :

10.3791/56333-v

November 23rd, 2017

•

12,177 Views

Marta H. Kubala¹^,², Yves A. DeClerck¹^,²^,³

¹Division of Hematology, Oncology and Blood and Marrow Transplantation, Department of Pediatrics, University of Southern California, ²The Saban Research Institute of Children's Hospital Los Angeles, ³Department of Biochemistry and Molecular Medicine, University of Southern California

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Conditional Gene Knockdown

Cancer Cell Lines

Monocyte Recruitment

Macrophage Recruitment

Tumor Microenvironment

Lentiviral Transduction

HEK 293 Cells

HCT 116 Cells

MDA MB 231 Cells

Monocyte Purification

Puromycin Selection

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