Positioning of Suction Electrode on Cranial Nerve to Evoke Eye Movement
7:58
Stimulation of Cranial Nerve
9:29
Results: Example of Pupil Constriction Evoked by Stimulating the Oculomotor Nerve (nIII)in the Whole-Head Preparation
10:15
Conclusion
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The overall goal of this electrophysiological protocol is tho provide the ability to measure the kinematics of eye movements of the red-eared slider turtle using an in vitro isolated head preparation. This method can help answer key questions in t
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This protocol describes how to use an in vitro isolated turtle head preparation to measure the kinematics of their eye movements. After removal of the brain from the cranium, cranial nerves can be stimulated with currents to quantify rotations of the eye and changes in pupil sizes.