Preparation of Lysate of Metabolically Labeled and UV Cross-linked Cells
3:33
Preparation of Samples for RNA-interactome Capture
5:13
RNA-interactome Capture
7:45
Results: Quality Control of Captured RNA-binding Proteins
8:53
Conclusion
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This method, CARIC, can help answer key questions in the field of post-transcription of gene recreation by providing a comprehensive census of RNA-binding proteins. The main advantage of CARIC is its ability to capture proteins bound to various ki
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A detailed protocol for applying the click chemistry-assisted RNA-interactome capture (CARIC) strategy to identify proteins binding to both coding and noncoding RNAs is presented.