June 21st, 2019
•Here we present a sensitive, rapid, and discriminating post-gel staining method to image RNAs tagged with RNA Mango aptamers I, II, III, or IV, using either native or denaturing polyacrylamide gel electrophoresis (PAGE) gels. After running standard PAGE gels, Mango-tagged RNA can be easily stained with TO1-Biotin and then analyzed using commonly available fluorescence readers.
Tags
Videos relacionados
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
A Colorimetric Method for Measuring Iron Content in Plants
Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs
Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
Rapid and Cost-Effective RNA Extraction of Rat Pancreatic Tissue
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados