Results: Post-gel Staining Method to Image RNAs Tagged with RNA Mango Aptamers
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Conclusion
Transcription
The Mango-tagged gel imaging methodology demonstrated here is robust and is anticipated to be able to be simply extended in terms of sensitivity and specificity. This would further simplify the routine purification of biologically important RNAs a
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Here we present a sensitive, rapid, and discriminating post-gel staining method to image RNAs tagged with RNA Mango aptamers I, II, III, or IV, using either native or denaturing polyacrylamide gel electrophoresis (PAGE) gels. After running standard PAGE gels, Mango-tagged RNA can be easily stained with TO1-Biotin and then analyzed using commonly available fluorescence readers.