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JoVE Journal

Behavior

This content is Open Access.

Two Different Real-Time Place Preference Paradigms Using Optogenetics within the Ventral Tegmental Area of the Mouse

12.8K Views

05:50 min

February 12th, 2020

February 12th, 2020

12,848 Views

0:04

Introduction

0:36

Setting up the Control of the Laser Source

1:44

Setting Up the Experiment for the RT-PP (Real-Time Place Preference) Approach and NCP (Neutral Compartment Preference) Approach

3:13

Performing an Experiment Using Laser Stimulation

4:24

Results: Real-Time Place Preference Experiment

5:18

Conclusion

Transcribir

Here we present two easy-to-follow step-by-step protocols for place preference paradigms using optogenetics in mice. Using these two different setups, preference and avoidance behaviors can be solidly assessed within the same apparatus with high spatial and temporal selectivity, and in a straightforward manner.

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