Dye-modification of Elastin-like Proteins via SPAAC
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THF Swelling Protocol
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BuOH Extrusion Protocol
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Results: THF Swelling Method and the BuOH Extrusion Method for the Guided Self-assembly of Amphiphilic ELPs into Supramolecular Structures
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Conclusion
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The efficient assembly of supramolecular structures for minimal cell design and drug delivery remains challenging. Here, we demonstrate efficient protocols that yield supramolecular structures based on amphiphilic elastin-like proteins. The presen
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At the interface of organic and aqueous solvents, tailored amphiphilic elastin-like proteins assemble into complex supramolecular structures such as vesicles, fibers and coacervates triggered by environmental parameters. The described assembly protocols yield Protein Membrane-Based Compartments (PMBCs) with tunable properties, enabling the encapsulation of various cargo.