Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro

5.8K Views

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10:01 min

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April 8th, 2020

DOI :

10.3791/60935-v

April 8th, 2020

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Andreas Schreiber*¹^,², Lara G. Stühn*¹^,², Süreyya E. Geissinger¹^,², Matthias C. Huber¹^,², Stefan M. Schiller¹^,²^,³^,⁴^,⁵^,⁶

¹Center for Biological Systems Analysis, University of Freiburg, ²Faculty of Biology, University of Freiburg, ³Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, ⁴BIOSS Centre for Biological Signalling Studies, University of Freiburg, ⁵IMTEK Department of Microsystems Engineering, University of Freiburg, ⁶Cluster of Excellence livMatS at FIT - Freiburg Center for Interactive Materials and Bioinspired Technologies, University of Freiburg

Summary

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Supramolecular Structures

Elastin like Proteins

Fluorescent Cargo Molecules

E coli Expression Culture

IPTG Induction

Unnatural Amino Acids

TRNA Synthetase

Lysis Buffer

Centrifugation

Chapters in this video

0:05

Introduction

0:45

Protein Expression and Preparation

3:38

Dye-modification of Elastin-like Proteins via SPAAC

4:44

THF Swelling Protocol

6:29

BuOH Extrusion Protocol

7:19

Results: THF Swelling Method and the BuOH Extrusion Method for the Guided Self-assembly of Amphiphilic ELPs into Supramolecular Structures

9:22

Conclusion

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