There is currently no gold standard for analyzing tail-vein injection induced metastatic lung tumor burdens. We utilized digital image analysis to accurately and reproducibly quantify lung metastases in a highly efficient manner. This method is ti
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Intravenous injection of cancer cells is often used in metastasis research, but the metastatic tumor burden can be difficult to analyze. Herein, we demonstrate a tail-vein injection model of metastasis and include a novel approach to analyze the resulting metastatic lung tumor burden.