March 4th, 2022
•The present protocol describes aortic cannulation and retrograde perfusion of the ex-vivo neonatal murine heart. A two-person strategy, using a dissecting microscope and a blunted small gauge needle, permits reliable cannulation. Quantification of longitudinal contractile tension is achieved using a force transducer connected to the apex of the left ventricle.
Videos relacionados
NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts
Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique
Intramyocardial Cell Delivery: Observations in Murine Hearts
Induction and Assessment of Ischemia-reperfusion Injury in Langendorff-perfused Rat Hearts
Neonatal Murine Cochlear Explant Technique as an In Vitro Screening Tool in Hearing Research
Optocardiography and Electrophysiology Studies of Ex Vivo Langendorff-perfused Hearts
Advanced Cardiac Rhythm Management by Applying Optogenetic Multi-Site Photostimulation in Murine Hearts
Creating Radio-cephalic Arteriovenous Fistula in the Forearm with a Modified No-Touch Technique
A Modified Simple Method for Induction of Myocardial Infarction in Mice
Modified Posterior Vertebral Column Resection for Patients with Thoracolumbar Kyphotic Deformity
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados