<meta charset="utf8"></head><body>Protocolo para el cocultivo de cianobacterias secretoras de sacarosa con socios fúngicos

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr >
			<td >10x Ph1 objective</td>
			<td >Carl Zeiss AG</td>
			<td >46 04 01-9904</td>
			<td ></td>
		</tr>
		<tr >
			<td >525/40 nm Bandpass Filter</td>
			<td >Beckman Coulter, Inc.</td>
			<td >A01-1-0051</td>
			<td>FITC filter (in 488 nm laser)</td>
		</tr>
		<tr >
			<td >660/10 nm Bandpass Filter</td>
			<td >Beckman Coulter, Inc.</td>
			<td>A01-1-0055</td>
			<td>APC channel (in 638 nm laser)</td>
		</tr>
		<tr >
			<td >710/50 nm Bandpass Filter</td>
			<td >Beckman Coulter, Inc.</td>
			<td>B71092</td>
			<td>PC5.5 channel (in 561 nm laser)</td>
		</tr>
		<tr >
			<td >Accuvettes for Multisizer 4e</td>
			<td>Beckman Coulter, Inc.</td>
			<td>A35473</td>
			<td>Sample cups with lids</td>
		</tr>
		<tr >
			<td >Aperture tube 30 &#956;M for Multisizer 4e</td>
			<td>Beckman Coulter, Inc.</td>
			<td>C92760</td>
			<td>Capillary</td>
		</tr>
		<tr >
			<td >Autoclave</td>
			<td >Lab Associates</td>
			<td >EQM-ARBP</td>
			<td>for sterilisation of material</td>
		</tr>
		<tr >
			<td >Baffled flasks ROTILABO&#174;, Straight neck, 250 ml</td>
			<td >Carl Roth GmbH + Co. KG</td>
			<td >LY95.1</td>
			<td></td>
		</tr>
		<tr >
			<td >BG11 / CoYBG11 components</td>
			<td>different suppliers</td>
			<td ></td>
			<td >see Table 1&#160;</td>
		</tr>
		<tr >
			<td >Bottle top sterile filter</td>
			<td >Sigmaaldrich</td>
			<td >Z222593</td>
			<td>to sterile media if you dont want to use autoclave</td>
		</tr>
		<tr >
			<td >Capillary 45 &#181;m</td>
			<td >Omni Life Science</td>
			<td>OLS 5651738</td>
			<td></td>
		</tr>
		<tr >
			<td >CASY Cell Counter Model TTC</td>
			<td>Schaerfe Systems</td>
			<td ></td>
			<td></td>
		</tr>
		<tr >
			<td >CASY cups</td>
			<td >Omni Life Science</td>
			<td>OLS 5651794</td>
			<td></td>
		</tr>
		<tr >
			<td >CASYclean</td>
			<td >Omni Life Science</td>
			<td>OLS 5651786</td>
			<td>Cleaning solution</td>
		</tr>
		<tr >
			<td >CASYton solution</td>
			<td >Omni Life Science</td>
			<td>OLS 5651808</td>
			<td>Isotonic solution&#160;</td>
		</tr>
		<tr >
			<td >Centrifuge tubes (50 mL)</td>
			<td >Sigmaaldrich</td>
			<td >CLS430828</td>
			<td></td>
		</tr>
		<tr >
			<td >CO2</td>
			<td >BOC</td>
			<td >270182-L</td>
			<td>to supply cyanobacteria axenic cultures and co-cultures</td>
		</tr>
		<tr >
			<td >CONTRAD&#174; 70</td>
			<td >Beckman Coulter, Inc.</td>
			<td>81911</td>
			<td>Deep clean solution</td>
		</tr>
		<tr >
			<td >Cover glass 20 &#215; 26 x 0.4 mm</td>
			<td >VWR International GmbH</td>
			<td>631-1190</td>
			<td>Cover glass for counting chamber</td>
		</tr>
		<tr >
			<td >Cuvettes</td>
			<td>Sarstedt&#160;</td>
			<td>67.742</td>
			<td></td>
		</tr>
		<tr >
			<td >Cyanobacteria BG-11 Freshwater Solution</td>
			<td >Merck KGaA</td>
			<td>C3061</td>
			<td></td>
		</tr>
		<tr >
			<td >CytExpert Software v. 2.6</td>
			<td >Beckman Coulter, Inc.</td>
			<td ></td>
			<td>Cytometer application software</td>
		</tr>
		<tr >
			<td >CytoFLEX Sheath Fluid</td>
			<td >Beckman Coulter, Inc.</td>
			<td >B51503</td>
			<td>Sheat fluid</td>
		</tr>
		<tr >
			<td >Cytometer CytoFLEX S</td>
			<td >Beckman Coulter, Inc.</td>
			<td >BE51180</td>
			<td></td>
		</tr>
		<tr >
			<td >DxH Cleaner for Multisizer 4e</td>
			<td>Beckman Coulter, Inc.</td>
			<td>628022</td>
			<td>Cleaning solution</td>
		</tr>
		<tr >
			<td >Eppendorf tube centrifuge (small)</td>
			<td >Labstac</td>
			<td >CEN16-15</td>
			<td>to centrifuge less than 2mL of culture</td>
		</tr>
		<tr >
			<td >Ethanol</td>
			<td >Carl Roth GmbH + Co. KG</td>
			<td >K928.1</td>
			<td></td>
		</tr>
		<tr >
			<td >FACS tubes</td>
			<td >Beckman Coulter, Inc.</td>
			<td >2523749</td>
			<td>tubes for semi-automatic mode</td>
		</tr>
		<tr >
			<td >FlowClean Cleaning Agent, 500ml</td>
			<td >Beckman Coulter, Inc.</td>
			<td>C48093</td>
			<td>Daily clean solution</td>
		</tr>
		<tr >
			<td >Graduated cylinders</td>
			<td >Sigmaaldrich</td>
			<td >Z131121</td>
			<td>for preparation of media</td>
		</tr>
		<tr >
			<td >HEPPSO</td>
			<td >Merck KGaA</td>
			<td>R426725</td>
			<td></td>
		</tr>
		<tr >
			<td >Infors MultiTron photoincubator</td>
			<td >Infors AG</td>
			<td ></td>
			<td>white LED light</td>
		</tr>
		<tr >
			<td >IPTG</td>
			<td >Merck KGaA</td>
			<td >I6758</td>
			<td>Prepare aliquots, store at -20&#176;C, thaw and keep on ice&#160;</td>
		</tr>
		<tr >
			<td >Isoton II for Multisizer 4e</td>
			<td>Beckman Coulter, Inc.</td>
			<td>8448011</td>
			<td>Isotonic solution&#160;</td>
		</tr>
		<tr >
			<td >Kaluza v. 2.2</td>
			<td >Beckman Coulter, Inc.</td>
			<td >B16406</td>
			<td>Cytometry data analysis software</td>
		</tr>
		<tr >
			<td >KimWipes</td>
			<td >VWR International GmbH</td>
			<td >115-2221</td>
			<td>Lint-free tissues</td>
		</tr>
		<tr >
			<td >KOH</td>
			<td >Merck KGaA</td>
			<td >221473</td>
			<td></td>
		</tr>
		<tr >
			<td >KPO3</td>
			<td >Noah Chemicals</td>
			<td>7790-53-6</td>
			<td></td>
		</tr>
		<tr >
			<td >Measuring beakers</td>
			<td >VWR</td>
			<td >213-3747</td>
			<td></td>
		</tr>
		<tr >
			<td >Micro test plate, 96 well, slip-on lid, flat base, PS, transparent</td>
			<td >SARSTEDT AG &#38; Co. KG</td>
			<td >82.1581.001</td>
			<td></td>
		</tr>
		<tr >
			<td >Microscope base</td>
			<td >Carl Zeiss AG</td>
			<td >47 09 18-9902/16</td>
			<td></td>
		</tr>
		<tr >
			<td >Microsope head</td>
			<td >Carl Zeiss AG</td>
			<td>47 30 14</td>
			<td></td>
		</tr>
		<tr >
			<td >Milli-Q water purification system</td>
			<td >Merck Millipore</td>
			<td>C85358</td>
			<td></td>
		</tr>
		<tr >
			<td >Mulitisizer 4e particle counter</td>
			<td>Beckman Coulter, Inc.</td>
			<td>B23005</td>
			<td>Alternative to the CASY Cell Counter</td>
		</tr>
		<tr >
			<td >Neubauer improved, depth 0.02 mm</td>
			<td>Assistent Germany</td>
			<td></td>
			<td>Counting chamber</td>
		</tr>
		<tr >
			<td >Neubauer improved, depth 0.1 mm</td>
			<td >Paul Marienfeld GmbH &#38; Co. KG</td>
			<td >640010</td>
			<td>Counting chamber</td>
		</tr>
		<tr >
			<td >Ready to Use Daily QC Fluorospheres</td>
			<td >Beckman Coulter, Inc.</td>
			<td >C65719</td>
			<td>Reference for QC</td>
		</tr>
		<tr >
			<td >SCHOTT bottles</td>
			<td >Dutscher</td>
			<td >90347</td>
			<td>for storage</td>
		</tr>
		<tr >
			<td >Specord&#174;200 Plus Spectrophotometer</td>
			<td>Analytic Jena GmbH</td>
			<td ></td>
			<td>OD600/750</td>
		</tr>
		<tr >
			<td >Sucrose</td>
			<td >Merck KGaA</td>
			<td>84100</td>
			<td></td>
		</tr>
		<tr >
			<td >Table top centrifuge (big)</td>
			<td >Labstac</td>
			<td >CEN18-06R</td>
			<td>collect culture biomass</td>
		</tr>
		<tr >
			<td >Yeast Nitrogen Base, without amino acids, without ammonium sulfate</td>
			<td >Thermo Fisher Scientific Inc.</td>
			<td >11743014</td>
			<td></td>
		</tr>
	</tbody>
</table>

assembly and quantification of co-cultures combining heterotrophic yeast with phototrophic sugar-secreting cyanobacteria

<meta charset="utf8"></head><body>Ensamblaje y cuantificación de cocultivos que combinan levaduras heterótrofas con cianobacterias fototróficas secretoras de azúcar

Ensamblaje y cuantificación de cocultivos que combinan levaduras heterótrofas con cianobacterias fototróficas secretoras de azúcar

With the increasing demand for sustainable biotechnologies, mixed consortia containing a phototrophic microbe and heterotrophic partner species are being ...

assembly-quantification-co-cultures-combining-heterotrophic-yeast

Research

JoVE Journal

Bioengineering

Assembly and Quantification of Co-Cultures Combining Heterotrophic Yeast with Phototrophic Sugar-Secreting Cyanobacteria

514 vistas.  Heinrich Heine University Düsseldorf. Este protocolo proporciona una guía completa para la configuración y el seguimiento cuantitativo de cocultivos, incluidas las cianobacterias fotoautótrofas secretoras de azúcar y las levaduras heterótrofas. 