Results: S. elongatus UTEX 2973 and Synechocystis PCC 6803
8:03
Conclusion
Transcripción
Cyanobacteria are an important phylum of photosynthetic microbes that are increasingly recognized as useful platforms for synthetic biology and renewable biotechnology applications. So developing robust techniques to simplify the design, cloning,
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Here, we present a protocol describing how to i) assemble a self-replicating vector using the CyanoGate modular cloning toolkit, ii) introduce the vector into a cyanobacterial host by conjugation, and iii) characterize transgenic cyanobacteria strains using a plate reader or flow cytometry.