December 29th, 2009
•We describe a streamlined protocol for generating "fillet" preparations of Drosophila embryos of specific genotypes. This protocol allows efficient execution of a variety of genetic screens. It also allows excellent visualization of structures in the late embryo.
Vidéos Connexes
Dissection of Drosophila Ovaries Video (Video) | JoVE
Dissection of 6.5 dpc Mouse Embryos Video (Video) | JoVE
Method for Whole Mount Antibody Staining in Chick Video (Video) | JoVE
Preparation of Drosophila Polytene Chromosome Squashes for Antibody Labeling Video (Video) | JoVE
In-vivo Centrifugation of Drosophila Embryos Video (Video) | JoVE
Upright Imaging of Drosophila Embryos Video (Video) | JoVE
RNAi Interference by dsRNA Injection into Drosophila Embryos Video (Video) | JoVE
Dissection and Staining of Drosophila Larval Ovaries Video (Video) | JoVE
Preparing Individual Drosophila Egg Chambers for Live Imaging Video (Video) | JoVE
Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos Video (Video) | JoVE