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Ocular Tissue Embedding and Cryosectioning in Optimal Cutting Temperature Compound for Fluorescence Imaging

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02:29 min

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November 10th, 2023

DOI :

10.3791/201613-v

November 10th, 2023

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Erik A. Souverein¹, Aaron Nagiel²^,³^,⁴, Ksenia Gnedeva⁵^,⁶

¹Keck School of Medicine, University of Southern California, ²The Vision Center, Department of Surgery, Children’s Hospital Los Angeles, ³The Saban Research Institute, Children’s Hospital Los Angeles, ⁴USC Roski Eye Institute, Department of Ophthalmology, Keck School of Medicine, University of Southern California, ⁵Eli and Edythe Broad CIRM Center for Regenerative Medicine and Stem Cell Research, University of Southern California, ⁶Tina and Rick Caruso Department of Otolaryngology-Head and Neck Surgery, University of Southern California

Transcription

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Ocular Tissue Embedding

Optimal Cutting Temperature Compound

Fluorescence Imaging

Cryo Protection

Sucrose Solution

Dissection Dish

Dissecting Microscope

Retinal Cryosection

Retinal Pigment Epithelium

Photoreceptor Layer

Bright field Image

Retinal Morphology

De la série

Rabbit Eye Dissection and Cryosectioning for Immunohistochemistry Studies

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