Cell Culture and Sorting of Differentiated Cardiomyocytes
4:50
Collection of Reaggregated Cardiac Cells
6:32
Generation of Human Engineered Cardiac Tissues
9:45
Results: Contractile Force Measurement Using Human Engineered Cardiac Tissues
11:05
Conclusion
Transcription
The overall goal of this procedure is to create human engineered cardiac tissues of a defined cellular composition. This method can address key challenges in the cardiac tissue engineering field, including controlling for variability and cardiac d
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This manuscript describes the creation of defined engineered cardiac tissues using surface marker expression and cell sorting. The defined tissues can then be used in a multi-tissue bioreactor to investigate mechanisms of cardiac cell therapy in order to provide a functional, yet controlled, model system of the human heart.