One-dimensional DNA Barcode Slides: Preparing the PDMS Barcode Mold
2:21
One-dimensional DNA Barcode Slides: Patterning of Poly-L-lysine (PLL)
3:39
One-dimensional DNA Barcode Slides: Patterning of ssDNA
5:00
Two-dimensional 3x3 DNA Arrays: Preparing the PDMS Barcode Mold and Flow Patterning of ssDNA
6:17
Two-dimensional 3x3 DNA Arrays: Validation and Immunoassay
7:32
Results: Multiplex-protein Detection from Single-cell Preparations
8:25
Conclusion
Transcription
The overall goal of this methodology is to fabricate an antibody array with applications in single-cell analysis. This method can help answer key questions in the field of single-cell analysis such as how can we detect secret proteins and intracel
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This protocol outlines the fabrication of a large-scale, multiplexed two-dimensional DNA or antibody array, with potential applications in cell signaling studies and biomarker detection.