The overall goal of this procedure is to isolate exosomes present in plasma of NSCLC patients that are free of non-exosomal micro RNA sources like free circulating micro RNAse using a double enzymatic treatment prior to exosome isolation. This met
Connectez-vous ou commencez votre essai gratuit pour accéder à ce contenu.
This protocol describes the feasibility to perform miRNA profiling in exosomes, released in plasma of NSCLC patients, through a commercial exosome isolation kit with Proteinase K and RNAse treatments, in order to avoid circulating miRNAs contamination and evaluate their biomarker features in NSCLC.