Exosomal miRNA Analysis in Non-small Cell Lung Cancer (NSCLC) Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

11.0K Views

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08:49 min

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May 27th, 2016

DOI :

10.3791/53900-v

May 27th, 2016

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Marco Giallombardo*¹^,², Jorge Chacártegui Borrás*²^,³, Marta Castiglia⁴, Nele Van Der Steen³, Inge Mertens⁵^,⁶, Patrick Pauwels⁷^,³, Marc Peeters⁸^,³, Christian Rolfo²^,³

¹Department of Biopathology and Medical Biotechnology, Section of Biology and Genetics, University of Palermo, ²Phase I-Early Clinical Trials Unit, Oncology Department, Antwerp University Hospital (UZA), ³Center for Oncological Research (CORE), Antwerp University, ⁴Department of Surgical, Oncological and Oral Sciences, Section of Medical Oncology, University of Palermo, ⁵Flemish Institute for Technological Research (VITO), ⁶CORE, Campus Groenenborger, Antwerp University, ⁷Molecular Pathology, Pathology Department, Antwerp University Hospital (UZA), ⁸Oncology Department, Antwerp University Hospital (UZA)

Summary

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Keywords Exosomal MiRNA

Reverse Transcription

Chapters in this video

0:05

Title

0:49

Exosome Isolation from Plasma Samples

3:11

Reverse Transcription of Exosomal miRNAs

4:25

RT-PCR Analysis of Exosomal miRNAs

6:43

Results: miRNA Analysis from NSCLC Patient Plasma

7:43

Conclusion

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