Organoid Plating for Pathogen Associated Molecular Patters (PAMP) and L. monocytogenes Challenge
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Results: Representative Mouse Intestinal Organoid Culture and Stimulation
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Conclusion
Transcription
The overall goal of this procedure is to measure the effects of pathogenic challenge on small intestine organoids with an emphasis on normalization techniques against the total cell number.This method could help answer key questions in the innate
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Here, a protocol to harvest, maintain, and treat mouse small intestinal organoids with pathogen associated molecular patterns (PAMPs) and Listeria monocytogenes is described, as well as emphasis on gene expression and proper normalization techniques for protein.