Isolation of HDL by Density Gradient Ultracentrifugation
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Desalting and Concentration of Lipoprotein Fractions
6:55
Results
8:38
Conclusion
Transcription
The overall goal of this method is to develop a simplified approach to rapidly isolate purified high density lipoproteins from human blood plasma for micro RNA analysis. This method can help answer key questions in hepatology and cardiology, such
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MicroRNAs play an important regulatory role and are emerging as novel therapeutic targets for various human diseases. It has been shown that miRNAs are carried in high density lipoproteins. We have developed a simplified method to rapidly isolate purified HDL suitable for miRNA analysis from human plasma.