Preparing Streptavidin Coated Particles and Addition of Capture Probe DNA
2:45
Hybridizing Complementary DNA to the Capture Probe-particles
3:46
TRPS Setup
4:58
Calibrating the Nanopore for Zeta Analysis and Running a Sample
6:43
Results: Size and Zeta Potential of Streptavidin Coated Nanoparticles by TRPS
7:47
Conclusion
Transcription
The overall goal of this procedure is to characterize the surface chemistry of nano particles using tunable resistant post sensing, or TRPS, and determine the zeta potential of the material. This is demonstrating by characterizing DNA modified nan
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Here we use a polyurethane tunable nanopore integrated into a resistive pulse sensing technique to characterize nanoparticles surface chemistry via the measurement of particle translocation velocities, which can be used to determine the zeta potential of individual nanoparticles.