Results: Bright-field Image and Pluripotency of iPSCs Generated from PBMCs
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Conclusion
Transcription
The overall goal of this study is to reprogram peripheral blood mononuclear cells into induced pluripotent stem cells, using serial plating by centrifugation. Originally, iPSCs, were generated by reprogramming fibroblasts. Today, various are used
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We propose a protocol for reprogramming peripheral blood mononuclear cells (PBMCs) into induced pluripotent stem cells (iPSCs). By plating the transduced blood cells onto matrix-coated plates with centrifugation, iPSCs are successfully induced from floating cells. This technique suggests a simple and effective reprogramming protocol for cells such as PBMCs and CBMCs.