Staining and Identification of Contaminants and Switchers
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Results: Induced Double-strand DNA Breaks Increase the Frequency of Switchers in a Population
9:01
Conclusion
Transcription
The overall goal of this method is to isolate trypanosomes expressing a particular variant surface glycoprotein or VSG from the rest of the population and identify parasites that have switched from the starting dominant VSG to any other VSG. This
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African trypanosomes grown in vitro undergo antigenic variation at a low rate, such that populations are made up of parasites expressing a dominant variant surface glycoprotein (VSG) type and a small population of "switched" variants. This protocol describes a fast method for detecting and quantifying these populations.