Results: Cell Counting Accuracy and Value of Calibration
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Conclusion
Transcription
The overall goal of this protocol is to use digital analysis tools to quickly make accurate counts of cells in a suspension or on a migration in an invasion assay membrane. This protocol can help researchers quantify cell number required for commo
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This paper describes the quantification of hemocytometer and migration/invasion micrographs through two new open-source ImageJ plugins Cell Concentration Calculator and migration assay Counter. Furthermore, it describes image acquisition and calibration protocols as well as discusses in detail the input requirements of the plugins.