Results: Cell Counting Accuracy and Value of Calibration
10:12
Conclusion
Transcript
The overall goal of this protocol is to use digital analysis tools to quickly make accurate counts of cells in a suspension or on a migration in an invasion assay membrane. This protocol can help researchers quantify cell number required for commo
Sign in or start your free trial to access this content
This paper describes the quantification of hemocytometer and migration/invasion micrographs through two new open-source ImageJ plugins Cell Concentration Calculator and migration assay Counter. Furthermore, it describes image acquisition and calibration protocols as well as discusses in detail the input requirements of the plugins.