Glycogen Precipitation, Enzymatic Hydrolysis, and Glycogen Determination
4:30
Determination of Total Glucose Content by the Glucose Oxidase-peroxidase (GOD-POD) Reagent
5:30
Results: Measurement of Glycogen Contents in Synechocystis sp.
6:15
Conclusion
Transcription
The overall goal of this procedure is to determine the glycogen content in cyanobacteria using an enzyme-based selective hydrolysis and assay. This method can help answer a key question in the cyanobacteria field, such as physiology, molecular gen
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Here, we present a reliable and easy assay to measure the glycogen content in cyanobacterial cells. The procedure entails precipitation, selectable depolymerization, and the detection of glucose residues. This method is suitable for both wildtype and genetically engineered strains and can facilitate the metabolic engineering of cyanobacteria.