siRNA Transient HIV-Tat-interactive Protein 60 kDa (TIP60) Depletion
2:08
Wound-healing Assay Cell Seeding and TIP60 Knockdown Efficiency Evaluation
4:10
Wound Creation, Imaging, and Single Cell Movement Tracking
6:32
Results: Representative Videos and Quantifications of siTIP60 and siControl Cell Migration
7:49
Conclusion
Transcription
The overall goal of this procedure is to observe the effects of the depletion of specific genes of interest on cell migration. This method can help us answer key questions in cancer metastasis, such as cancer cell migration. The main advantage of
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Here, we present the real-time monitoring of cell migration in a wound-healing assay using TIP60-depleted MCF10A breast epithelial cells. The implementation of live-cell imaging techniques in our protocol allows us to analyze and visualize single-cell movement in real time and across time.