Liquid Culture for Treatment with RNAi Starting at L1
2:40
Collection of Young and Aged Worms Subjected to RNAi in Liquid Culture
6:14
Disruption of Animals
6:58
Isolation of SDS-insoluble Proteins for Mass Spectrometry
10:03
Results: Reduced IIS Prevents Age-dependent Protein Aggregation
11:26
Conclusion
Transcription
The overall goal of this experiment is to detect changes in protein insolubility with age in C.elegans and to evaluate how targeted gene knockdown influences this aging marker. This method can help answer key questions in the field of aging and pr
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The goal of the method presented here is to explore protein aggregation during normal aging in the model organism C. elegans. The protocol represents a powerful tool to study the highly insoluble large aggregates that form with age and to determine how changes in proteostasis impact protein aggregation.