RT-LAMP for Detection of Zika-infected Mosquitoes Using Q-paper Technology
2:23
Testing Lyophilized RT-LAMP Reagents on Urine Samples Containing Zika
4:05
Results: Singleplex and Multiplex RT-LAMP for Detection of Zika, Chikungunya, and Dengue 1
5:40
Conclusion
Transcription
The overall goal of this procedure is to demonstrate the use of a multiplexed point-of-care diagnostics platform that does not require extensive sample preparation and expensive instrumentation and it's used in a variety of biological samples. Thi
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Current multiplexed diagnostics to detect Zika, chikungunya, and dengue viruses require complex sample preparation and expensive instrumentation, and are difficult to use in low resource environments. We show a diagnostic that uses isothermal amplification with target-specific strand displaceable probes to detect and differentiate these viruses with high sensitivity and specificity.