Sample Preparation for Calibration Curve and Method Validation
3:50
SM Analysis by LC-ESI-MS/MS
5:33
Batch File Creation and Assigning the MS Three Product Ion Spectra of Sphingomyelin
6:23
Quantitative Analysis of SM by LC-ESI-MS/MS
7:02
Results: Validating the Quantitative and Qualitative Method for Sphingomyelin
8:01
Conclusion
Transcription
The overall goal of this procedure is to precisely analyze the amount and the structure of each Sphingomyelin species in biological samples. This method can help answer key questions in the lipid field pertaining to lipid biology and lipid The mai
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Here, we present a protocol to quantify and qualify each sphingomyelin species using multiple reaction monitoring and MS/MS/MS mode, respectively.