Crosslinking of Embryonic Stem Cells for ChIP and CARIP
2:29
Chromatin Sonication for ChIP and CARIP
3:48
ChIP and CARIP Process
7:12
Double-stranded cDNA Synthesis for CARIP-Seq
8:53
Use of ChIP-Seq and CARIP-Seq to Study Global Protein-DNA Interactions and Chromatin-Associated RNAs in Embryonic Stem Cells
10:43
Conclusion
Transcription
The overall goal of CARIP-Seq is to survey genome-wide chromatin-associated RNAs. The goal of ChIP-Seq is to evaluate protein-DNA interactions on a global scale in embryonic stem cells. These methods can help answer key questions in the epigenetic
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Here, we describe methods to perform ChIP-Seq and CARIP-Seq, including library preparation for next-generation sequencing, to generate global epigenomic and chromatin-associated RNA maps in ES cells.