Preparation of Aluminum (Al) Substrate by Electropolishing
1:34
Fabrication of Gradient Anodic Aluminum Oxide (AAO) Mold with Phosphoric Acid Electrolyte
4:28
Deposition of Anti-Sticking Layer on Gradient AAO Mold with Self-Assembled Monolayer
5:46
Fabrication of Gradient Nanopattern Plates by Thermal Imprinting
7:08
Cultivation of hECFCs and Culture on the Gradient Nanopattern Plates
9:14
Results: Evaluation and Analysis of Culturing hECFCs on Fabricated Gradient Nanopattern Plates
10:37
Conclusion
Transcription
The main advantage of this technique is that it can produce gradient patterns with high reproducibility and allowed the screening of cell response to parasites observational structures. The response observes to physical stimulation is not yet full
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Here, we present a protocol for the fabrication of gradient nanopattern plates via thermal nanoimprinting and the method of screening responses of human endothelial progenitor cells to the nanostructures. By using the described technology, it is possible to produce a scaffold that can manipulate cell behavior by physical stimuli.